Fluorescent bioinspired albumin/polydopamine nanoparticles and their interactions with Escherichia coli cells

• Eloïse Equy,
• Jordana Hirtzel,
• Sophie Hellé,
• Béatrice Heurtault,
• Eric Mathieu,
• Morgane Rabineau,
• Vincent Ball and
• Lydie Ploux

Beilstein J. Nanotechnol. 2023, 14, 1208–1224, doi:10.3762/bjnano.14.100

• ) of poly(lactic-co-glycolic acid) (PLGA) [7], polycaprolactone [8], and chitosan [9]. Furthermore, fluorescent ONPs are a promising way to facilitate the localization of NPs in cells through fluorescence imaging. They can also be used for fluorescent labelling of cells, especially for live cell

• imaging, provided that the ONPs are harmless for cells. This has been developed for eukaryotic cells [10], but the use of ONPs for labelling bacterial cells is still rare and not described in literature for alive bacterial cells. The main limitation is probably the frequent cytotoxic effect of ONPs on

Curcumin-loaded albumin submicron particles with potential as a cancer therapy: an in vitro study

• Nittiya Suwannasom,
• Netsai Sriaksorn,
• Chutamas Thepmalee,
• Krissana Khoothiam,
• Ausanai Prapan,
• Hans Bäumler and
• Chonthida Thephinlap

Beilstein J. Nanotechnol. 2023, 14, 1127–1140, doi:10.3762/bjnano.14.93

• Cytation™ 5 Cell Imaging Multi-Mode Reader and analyzed using Gen5 data analysis software (BioTek® Instruments, Inc., Winooski, VT, USA). Particle size and zeta potential Hydrodynamic size, polydispersity index (PDI), and zeta potential of the particles were evaluated using a Zetasizer Nano ZS (Malvern

• the tetrazolium dye MTT to water-insoluble purple formazan crystals, indicating mitochondrial function and metabolically active cells. After medium removal, the dark blue crystals were dissolved in 100 μL of DMSO; then, the absorption was measured at wavelengths of 570 nm with a Cytation™ 5 Cell

• Imaging Multi-Mode Reader and analyzed using BioTek Gen5 software. Cellular uptake To investigate the uptake of particles, HSA-MPs and CUR-HSA-MPs (8%) were labeled with FITC at a ratio of 9:1 at RT for 1 h and protected from light. After incubation, the labeled particles were washed three times with

Recent advances in green carbon dots (2015–2022): synthesis, metal ion sensing, and biological applications

• Aisha Kanwal,
• Naheed Bibi,
• Sajjad Hyder,
• Arif Muhammad,
• Hao Ren,
• Jiangtao Liu and
• Zhongli Lei

Beilstein J. Nanotechnol. 2022, 13, 1068–1107, doi:10.3762/bjnano.13.93

• were employed as probes for detecting metal ions and in live-cell imaging, and they had a high quantum yield of 61% [126]. Chen et al. proposed a quick method for producing highly luminous CDs by hydrothermally treating lignin with H2O2. It is well recognized that under the photoassisted catalysis of

Detection and imaging of Hg(II) in vivo using glutathione-functionalized gold nanoparticles

• Gufeng Li,
• Shaoqing Li,
• Rui Wang,
• Min Yang,
• Lizhu Zhang,
• Yanli Zhang,
• Wenrong Yang and
• Hongbin Wang

Beilstein J. Nanotechnol. 2022, 13, 549–559, doi:10.3762/bjnano.13.46

• the fluorescent “ON” form. Keywords: cell imaging; fluorescence probes; glutathione; gold nanoparticles; mercury ions; rhodamine 6G derivatives; Introduction Metal nanoparticles have been widely used in the development and construction of sensor systems and drug carriers due to their excellent

• capabilities of the GSH-modified GNPs, additional modification strategies are needed. In this study, rhodamine 6G derivative conjugated to GSH-modified GNPs (GNPs-GSH-Rh6G2) was designed and synthesized in order to effectively tune the properties of GSH-functionalized GNPs for the detection of Hg2+ and cell

• imaging. We chose rhodamine 6G because of its excellent light stability, high fluorescence quantum yield, and good biocompatibility [36][37]. The functionalized GNPs have excellent selectivity for Hg2+. Furthermore, to evaluate the imaging effects of functionalized GNPs in cells, GNPs-GSH-Rh6G2 was

The role of convolutional neural networks in scanning probe microscopy: a review

• Ido Azuri,
• Irit Rosenhek-Goldian,
• Neta Regev-Rudzki,
• Georg Fantner and
• Sidney R. Cohen

Beilstein J. Nanotechnol. 2021, 12, 878–901, doi:10.3762/bjnano.12.66

• learning and linear programming (an optimization method) for tracking single cells in live-cell imaging of both fluorescent and bright-field images of the cell cytoplasm [112]. Newby et al. developed a CNN for fully automated submicrometer-scale localization of particles such as viruses, proteins, and drug

The nanomorphology of cell surfaces of adhered osteoblasts

• Christian Voelkner,
• Mirco Wendt,
• Regina Lange,
• Max Ulbrich,
• Martina Gruening,
• Susanne Staehlke,
• Barbara Nebe,
• Ingo Barke and
• Sylvia Speller

Beilstein J. Nanotechnol. 2021, 12, 242–256, doi:10.3762/bjnano.12.20

• ruffles moving beneath the pipette opening. Slightly higher rms amplitudes may also be due to different temperature because fixed cells are measured at room temperature, while live-cell imaging is carried out at 37 °C. Frequency response behavior Owing to the fact that morphological changes at the cell

Design of V-shaped cantilevers for enhanced multifrequency AFM measurements

• Mehrnoosh Damircheli and
• Babak Eslami

Beilstein J. Nanotechnol. 2020, 11, 1525–1541, doi:10.3762/bjnano.11.135

• researchers found that for biological or cell imaging, V-shape cantilevers are more appropriate than rectangular cantilevers due to higher torsional and lateral stability [13]. The higher stability of V-shaped cantilevers with a low normal spring constant provides a unique combination, which is not found in

Luminescent gold nanoclusters for bioimaging applications

• Nonappa

Beilstein J. Nanotechnol. 2020, 11, 533–546, doi:10.3762/bjnano.11.42

• rapid detection and the development of new immunoassays. Imaging and labeling mammalian cell lines Beyond their antibacterial effect and pathogen sensing, the surface functionalities of NCs allow for selective labeling for the detection of biomolecules, intracellular metal ion sensing, live-cell imaging

Small protein sequences can induce cellular uptake of complex nanohybrids

• Jan-Philip Merkl,
• Malak Safi,
• Christian Schmidtke,
• Fadi Aldeek,
• Johannes Ostermann,
• Tatiana Domitrovic,
• Sebastian Gärtner,
• John E. Johnson,
• Horst Weller and
• Hedi Mattoussi

Beilstein J. Nanotechnol. 2019, 10, 2477–2482, doi:10.3762/bjnano.10.238

• have also been discussed [12][14][15]. More recently, there have been a few reports discussing the use of luminescent Eu-loaded hydroxyapatite nanocrystals for rapid HeLa cancer cell imaging [9][11][16], or the nanostructure self‐assembly driven by amino acid coordination to increase the biological

Nitrogen-vacancy centers in diamond for nanoscale magnetic resonance imaging applications

• Alberto Boretti,
• Lorenzo Rosa,
• Jonathan Blackledge and
• Stefania Castelletto

Beilstein J. Nanotechnol. 2019, 10, 2128–2151, doi:10.3762/bjnano.10.207

• conditions with high spatial resolution. Another demonstration of ambient temperature in-cell imaging of biomagnetic nanostructures with resolution down to 400 nm has been given by layering magnetotactic bacteria on diamond with a superficial array of NV centers. Optical detection of quantum spin states

Gold-coated plant virus as computed tomography imaging contrast agent

• Alaa A. A. Aljabali,
• Mazhar S. Al Zoubi,
• Khalid M. Al-Batanyeh,
• Ali Al-Radaideh,
• Mohammad A. Obeid,
• Abeer Al Sharabi,
• Walhan Alshaer,
• Bayan AbuFares,
• Tasnim Al-Zanati,
• Murtaza M. Tambuwala,
• Naveed Akbar and
• David J. Evans

Beilstein J. Nanotechnol. 2019, 10, 1983–1993, doi:10.3762/bjnano.10.195

• −53.8 ± 2.4 mV upon antibody coating of the particles. The zeta potential value of VCAM1-PEG5000Au-CPMV becomes more negative [38], which is consistent with the literature [42]. In vitro fluorescent cell imaging Confocal fluorescence microscopy was performed on the cell lines to demonstrate the

Serum type and concentration both affect the protein-corona composition of PLGA nanoparticles

• Katrin Partikel,
• Robin Korte,
• Dennis Mulac,
• Hans-Ulrich Humpf and
• Klaus Langer

Beilstein J. Nanotechnol. 2019, 10, 1002–1015, doi:10.3762/bjnano.10.101

• with water instead of serum (PLGA NPs). We then added the NPs as well as the free dye Lumogen® Red to the cells and monitored the cell interaction under serum-free conditions over a time period of 24 h by live-cell imaging (Figure 7). The results revealed that the interaction between the unformulated

• after reaching 80–90% confluence or were used for cell culture experiments. Determination of nanoparticle–cell interaction by live-cell imaging In order to investigate the interaction between PLGA NPs displaying a protein corona of different characteristics and HepG2 cells an IncuCyte®S3 Live-Cell

Tungsten disulfide-based nanocomposites for photothermal therapy

• Tzuriel Levin,
• Hagit Sade,
• Rina Ben-Shabbat Binyamini,
• Maayan Pour,
• Iftach Nachman and
• Jean-Paul Lellouche

Beilstein J. Nanotechnol. 2019, 10, 811–822, doi:10.3762/bjnano.10.81

• [45], nanodots [40] and hollow spheres [46]. Recently, WS2 nanotubes functionalized with C-dots showed promising results for PTT and cell imaging [47]. We selected nanotubes for their mechanical properties and the possibility of coordinate bonds with sulfur atoms, which enables bonding with CAN-mag

Polydopamine-coated Au nanorods for targeted fluorescent cell imaging and photothermal therapy

• Boris N. Khlebtsov,
• Andrey M. Burov,
• Timofey E. Pylaev and
• Nikolai G. Khlebtsov

Beilstein J. Nanotechnol. 2019, 10, 794–803, doi:10.3762/bjnano.10.79

• -targeted PPT and chemotherapy (via Cu(II) release) [34]; AuNR-PDA-pMBA-Ab for targeted SERS cell imaging [31]; AuNR-PDA-MB/DOX for notargeted combined photodynamic and chemotherapy in vivo [32]; AuNR-PDA-Cisplatin-Iodine125-RGDpeptide for targeted MRI imaging and chemotherapy in vivo [33]. However, AuNR

• loading with rhodamine 123 makes the nanoparticles suitable for cell imaging with a simple fluorescent microscope; (3) through using NIR-mediated photothermal therapy the cancer cells can be killed with a high efficiency. Results and Discussion Synthesis and characterization of the AuNRs-PDA-R123-folate

• -light illumination due to the presence of R123 molecules. Additionally, nanoparticles can selectively accumulate in the cancer cells because of targeting to folate receptors. Folate-mediated cell imaging Efficient cellular uptake of nanocarriers is significant to ensure the therapeutic efficacy of

Ceria/polymer nanocontainers for high-performance encapsulation of fluorophores

• Kartheek Katta,
• Dmitry Busko,
• Yuri Avlasevich,
• Katharina Landfester,
• Stanislav Baluschev and
• Rafael Muñoz-Espí

Beilstein J. Nanotechnol. 2019, 10, 522–530, doi:10.3762/bjnano.10.53

• scattering than visible light, causes less photodamage, and can penetrate deeper into tissues. Hence, it is preferred for life-science applications. Organic dyes that can be excited above 600 nm are highly favorable for live-cell imaging experiments, because the background signal obtained from the

Surface plasmon resonance enhancement of photoluminescence intensity and bioimaging application of gold nanorod@CdSe/ZnS quantum dots

• Siyi Hu,
• Yu Ren,
• Yue Wang,
• Jinhua Li,
• Junle Qu,
• Liwei Liu,
• Hanbin Ma and
• Yuguo Tang

Beilstein J. Nanotechnol. 2019, 10, 22–31, doi:10.3762/bjnano.10.3

• @CdSe/ZnS can be used in cell imaging studies, after conjugation with FA, we performed a MCF-7 breast cancer cell targeting imaging study, and the results as shown in Figure 8. Robust cellular uptake could be obtained from the CdSe/ZnS@FA and GNR@CdSe/ZnS@FA treated samples. The obvious luminescence and

• plasmon resonance of GNRs. The experimental results correlated well with the theoretical simulation. The results of the biological detection study indicated that this nanomaterial is biocompatible and that there is significant PL signal for cell imaging. This research is promising for future nanophotonics

• polarization along the long axis direction z. d is the distance between the dipole source center and the GNR center, and the length of the GNRs was fixed at 40 nm. In vitro cell imaging study MCF-7 breast cancer cells (American Type Culture Collection) were cultured with Dulbecco’s Modified Eagle’s Medium

Enhanced antineoplastic/therapeutic efficacy using 5-fluorouracil-loaded calcium phosphate nanoparticles

• Shanid Mohiyuddin,
• Saba Naqvi and
• Gopinath Packirisamy

Beilstein J. Nanotechnol. 2018, 9, 2499–2515, doi:10.3762/bjnano.9.233

• corresponding to the different phases of apoptosis were captured in an inverted phase contrast fluorescent microscope (EVOS cell imaging system) using corresponding fluorescent filters. Hoechst 33342 and rhodamine B dual staining The combinatorial staining of rhodamine B and Hoechst 33342 were used as a tool to

Facile chemical routes to mesoporous silver substrates for SERS analysis

• Elina A. Tastekova,
• Alexander Y. Polyakov,
• Anastasia E. Goldt,
• Alexander V. Sidorov,
• Alexandra A. Oshmyanskaya,
• Irina V. Sukhorukova,
• Dmitry V. Shtansky,
• Wolgang Grünert and
• Anastasia V. Grigorieva

Beilstein J. Nanotechnol. 2018, 9, 880–889, doi:10.3762/bjnano.9.82

• long time in air. Mesoporous noble metals are mostly used as catalysts for high surface energy, gas sensor components, cell imaging mediators, etc. [5]. The most popular methods for mesoporous metal processing include acidic etching of bimetallic molts [6], electrochemical dealloying [7

Green synthesis of fluorescent carbon dots from spices for in vitro imaging and tumour cell growth inhibition

• Nagamalai Vasimalai,
• Vânia Vilas-Boas,
• Juan Gallo,
• María de Fátima Cerqueira,
• Mario Menéndez-Miranda,
• José Manuel Costa-Fernández,
• Lorena Diéguez,
• Begoña Espiña and
• María Teresa Fernández-Argüelles

Beilstein J. Nanotechnol. 2018, 9, 530–544, doi:10.3762/bjnano.9.51

• yield among all of the spice-derived C-dots. A summary of the characteristic parameters studied for each spice C-dot is collected in Table 1. Cell viability measurements and cell imaging using the C-dots Concentrations varying from 0.1 mg·mL−1 to 2 mg·mL−1 (and up to 4 mg·mL−1 in the case of black

• and its fluorescence was measured at 560 nm before and after incubation with growing concentrations (0.1, 0.5, 1.0 and 2.0 mg·mL−1) of each type of C-dots. Results are means ± SD of the percentage of initial fluorescence. Cell imaging Cells were seeded in 8-well glass bottom μ-slides (Ibidi

Involvement of two uptake mechanisms of gold and iron oxide nanoparticles in a co-exposure scenario using mouse macrophages

• Dimitri Vanhecke,
• Dagmar A. Kuhn,
• Dorleta Jimenez de Aberasturi,
• Sandor Balog,
• Ana Milosevic,
• Dominic Urban,
• Diana Peckys,
• Niels de Jonge,
• Wolfgang J. Parak,
• Alke Petri-Fink and
• Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2017, 8, 2396–2409, doi:10.3762/bjnano.8.239

• negative, must be considered for nanotechnology and nanomedicine in particular to develop to its full potential. Keywords: co-exposure; endocytosis; live cell imaging; nanoparticles; quantitative microscopy; Introduction Over the past two decades, improvements in nanomaterial research were followed by a

• . Uptake occurs in a “first come, first served” manner, as suggested by the uptake slopes seen in the live-cell imaging and supported by the ICP data. Also the TEM data did not find evidence for particle-specific sorting before the uptake. A distinct type of organelles, namely caveosomes [36], has been

• × phosphate-buffered saline (PBS). After 1 h of staining (in the dark at RT) and three washing cycles with 1× PBS the cells were mounted using Glycergel mounting media (C0563, Dako, Baar, Switzerland). For live-cell imaging, cells were seeded in a Lab-TekTM II chambered cover glass four-well chamber (1.5

Uptake and intracellular accumulation of diamond nanoparticles – a metabolic and cytotoxic study

• Antonín Brož,
• Lucie Bačáková,
• Pavla Štenclová,
• Alexander Kromka and
• Štěpán Potocký

Beilstein J. Nanotechnol. 2017, 8, 1649–1657, doi:10.3762/bjnano.8.165

• phase contrast live-cell imaging in real time. For both types of oxygen-terminated HPHT NDs, the cell viability and the cell number remained almost the same for concentrations up to 100 µg/mL within the whole range of ND diameters tested. The uptake of hydrogen-terminated detonation NDs caused the

• types. Keywords: cell viability; FTIR; live-cell imaging; MTS; nanodiamond; SAOS-2 cells; Introduction Carbon-based materials in the form of nanostructures are showing great promise as engineering and biomedical materials [1]. Moreover, diamond represents a new class of material with properties that

• viability and are negatively correlated with the material cytotoxicity. The live-cell imaging method was used for observing the intake of NDs into the cells. The results were evaluated on the basis of particle size, surface potential, surface functional groups, and the concentration of the ND suspension

Bright fluorescent silica-nanoparticle probes for high-resolution STED and confocal microscopy

• Isabella Tavernaro,
• Christian Cavelius,
• Henrike Peuschel and
• Annette Kraegeloh

Beilstein J. Nanotechnol. 2017, 8, 1283–1296, doi:10.3762/bjnano.8.130

• the number of incorporated dye molecules can be controlled by the amount of the modified dye and by growing non-fluorescent or fluorescent shells. Brightness and quantum yields Sensitive imaging applications, such as live-cell imaging, three-dimensional imaging or STED microscopy, require a high

Evaluation of quantum dot conjugated antibodies for immunofluorescent labelling of cellular targets

• Jennifer E. Francis,
• David Mason and
• Raphaël Lévy

Beilstein J. Nanotechnol. 2017, 8, 1238–1249, doi:10.3762/bjnano.8.125

• Jennifer E. Francis David Mason Raphael Levy Department of Biochemistry, Institute of Integrative Biology, Biosciences Building, Crown Street, Liverpool, L69 7ZB, United Kingdom Centre for Cell Imaging, Institute of Integrative Biology, Biosciences Building, Crown Street, Liverpool, L69 7ZB

• Bulinkski from Colombia University for the TC7 3xGFP cells and also appreciation to Professor Philip Rudland from University of Liverpool for supplying the rat mammary (Rama) 27 fibroblasts. We would like to acknowledge and thank the Liverpool Centre for Cell Imaging (CCI) for training and access to

On the pathway of cellular uptake: new insight into the interaction between the cell membrane and very small nanoparticles

• Claudia Messerschmidt,
• Daniel Hofmann,
• Anja Kroeger,
• Katharina Landfester,
• Volker Mailänder and
• Ingo Lieberwirth

Beilstein J. Nanotechnol. 2016, 7, 1296–1311, doi:10.3762/bjnano.7.121

• indicator for a necrosis-based cell death. Moreover, the live cell imaging with the detection of a burst event and the Hoechst staining of the cell nucleus points into the direction of necrosis. Despite of this common conclusion, we analyzed the cleavage of Caspase-3 showing no specific activation of major

• observations. The LDH assay (Figure 6) ranks SiNP-12 and SiNP-7 to be most toxic whereas life cell imaging (Supporting Information File 1, Figure S10) points at SiNP-12. From the ATP measurements (Figure 7) no clear ranking statement is possible. This confusion in correlation between NP size and cytotoxicity

Atomic force microscopy as analytical tool to study physico-mechanical properties of intestinal cells

• Christa Schimpel,
• Oliver Werzer,
• Eleonore Fröhlich,
• Gerd Leitinger,
• Markus Absenger-Novak,
• Birgit Teubl,
• Andreas Zimmer and
• Eva Roblegg

Beilstein J. Nanotechnol. 2015, 6, 1457–1466, doi:10.3762/bjnano.6.151

• the differentiation of M cells. Cell monolayer integrity and confluence were evaluated by measuring the transepithelial electrical resistance (TEER) with an Endohm culture cup connected to an epithelial volt ohm meter (World Precisions Instruments, Sarasota, USA). For AFM cell imaging/force